Solve Biochemistry AIIMS 2018 1. Which of the following does not require 5' capping? U6 snRNA tRNA of Alanine siRNA mRNA for Histone None 2. Vitamin K in its coenzyme form is regenerated by the enzyme Glutathione reductase Vitamin K reductase Quinone reductase Epoxide reductase None 3. Identify the phenomenon shown below Translation Transformation Complementation Conjugation None 4. An adolescent male patient presented with pain in the calf muscles on exercise. On biopsy, an excessive amount of glycogen was found to be present in the muscle. What is the most likely enzyme deficiency? Phosphofructokinase 1 Muscle glycogen phosphorylase Glucose 6 phosphatase Muscle-debranching enzyme None 5. A middle-aged woman presents with fissured tongue and peripheral neuropathy. On Investigations reduced glutathione reductase activity was found, Which vitamin deficiency is the likely cause of this? Vitamin B12 Vitamin B6 Vitamin B2 Vitamin B1 None 6. Which of the following enhances iron absorption? Vitamin C Thiamine Vitamin A Riboflavin None 7. Alkali disease of livestock is caused by an excess of which of the following minerals? Molybdenum Manganese Selenium Iron None 8. Which of the following does not favor euchromatin formation due to changes occurring at Cytosine residues at CpG islands in DNA? Alkylation Sumoylation Phosphorylation Methylation None 9. Which of the following techniques is based on RNA? Next generation sequencing Sanger's technique RT PCR Western blot None 10. Which of the following types of collagens is involved in wound healing Type IV Type III Type II Type V None 11. Vitamin C cannot be produced in humans due to lack of L-gulonolactone oxidase UDP glucose dehydrogenase Pyruvate dehydrogenase Xylitol reductase None 12. Klenow fragment lacks the activity of 5'->3' Exonuclease 3'->5' Exonuclease 5'->3' DNA polymerase 3'->5' DNA polymerase None 13. An infant presented to the OPD with a history of vomiting and poor feeding. Musty odor is present in the baby's sweat and urine. Guthrie test was done and it was found to be positive. All are true regarding this disease except White patch of hair due to tryptophan deficiency Phenylalanine hydroxylase enzyme defect Mental retardation is present Phenylacetate positive in urine None 14. A 12-year-old girl suffers from an inadequately treated type 1 diabetes mellitus. She is currently hyperglycemic. Which of the following is most likely to occur? Increased conversion of fatty acids to acetyl-CoA Increased protein synthesis Decreased cholesterol synthesis Glycogenesis in muscle None 15. In a reaction, the substrate is available in a concentration that is 1000 times the Km value of the enzyme. After 9 minutes of reaction, 1% of substrate is converted to product (12 microgram/ml). If the concentration of the enzyme is changed to 1/3 and concentration of substrate is doubled, what is the time taken to convert the substrate into the same amount of product that i.e. 12 microgram/ml? 4.5 minutes 27 minutes 13.5 minutes 9 minutes None 16. Child presents with hypotonia and seizures. It was confirmed to be Zellweger syndrome. Which among the following accumulates in brain in this condition? Glucose Long chain fatty acid Lactic acid Triglycerides None 17. What would be the respiratory quotient if someone is on an exclusive carbohydrate diet? 1.2 1 0.8 0.7 None 18. The best method to measure HbA1C is Isoelectric focusing Affinity chromatography lon exchange chromatography Electrophoresis None 19. Which of the following is the best screening investigation for metabolic disorders? Tandem mass spectrometry Western blot PCR Gel electrophoresis None 20. Which of the following enzyme activity decreases in fasting? Glycogen phosphorylase Pyruvate carboxylase Hormone-sensitive lipase Phosphofructokinase 1 None 21. Fluoroacetate is a component of pesticides. Which of the following metabolic pathways is inhibited by this compound? TCA cycle Glycolytic pathway Oxidative phosphorylation ETC None 1 out of 21 Time's up
