Solve Biochemistry INICET 2021 1. Which of the following is the best method to detect HbA1c? lon-exchange chromatography Affinity chromatography Immunoassay Electrophoresis None 2. Which of the following options is false in a patient with advanced liver disease? Decreased ATP synthesis Hyperammonemia Oxidative decarboxylation of alpha-keto glutarate Increased synthesis of glutamine and glutamate from alpha-ketoglutarate None 3. What is the effect of RNAi on gene expression? Knock out Knock up Knock down Knock in None 4. Which of the following is associated with a defect in collagen maturity? Beri-beri Scurvy Rickets Night blindness None 5. Methyl-tetrahydro-folic acid gets accumulated in deficiency of which of the following? B12 B2 B1 B6 None 6. Which of the following is not a substrate for glucose formation? Acetyl coenzyme A Glycerol Alanine Lactate None 7. Cytochrome oxidase is inhibited in all of the following poisonings except: Carbon monoxide Hydrogen sulfide Cyanide Methane None 8. Which of the following is the best test for quantitative analysis of an RNA sample? PCR-Restriction Fragment Length Polymorphism Real-time Polymerase chain reaction Reverse transcriptase PCR Reverse transcriptase-polymerase chain reaction followed by Real-time PCR None 9. Which of the following molecular analysis methods is used to detect RNA? G banding Sanger's method RT-PCR Western blot None 10. Wobble hypothesis explains which of the following concepts of the genetic code? Unambiguous Universal Non-punctuated Degeneracy None 11. Which of the following statements are true regarding mitochondrial DNA? Double-stranded circular DNA with about 16,000 base pairs A slight change in nucleotide sequence as compared to cellular DNA Higher rate of mutations as compared to the nuclear genome Encodes all electron transport chain proteins 1,3 1,2 1,2,3,4 Only 4 None 12. Insulin inhibits which of the following lipase enzymes? Lipoprotein lipase Pancreatic lipase Hormone-sensitive lipase Gastric lipase None 13. Glycated hemoglobin (HbA1c) is best measured using? Affinity chromatography Ion exchange chromatography Isoelectric focussing Electrophoresis None 14. A patient with thiamine deficiency develops lactic acidosis. Which of the following enzymes is affected? Pyruvate carboxylase Phosphoenolpyruvate carboxykinase Pyruvate dehydrogenase Transketolase None 15. What is the main substrate and metabolic fuel used by RBCs during fasting? Ketone bodies Glucose Free fatty acids Amino acids None 16. miRNA binds to which part of the mRNA to inhibit translation? Gene promoter Gene body 3' UTR 5' UTR None 17. Which of the following is not amphipathic? Sphingolipids Phosphoglycerol Triglycerides Glycolipids None 18. The enzyme kinetics depicted in the Graph is suggestive of- Cooperative Binding Allosteric Activation Michaelis Menten kinetics Sigmoid Curve None 19. Which of the following methods cannot be used to precipitate proteins? Add alcohol and acetone Using heavy metal ions Moving pH away from isoelectric pH Adding trichloroacetic acid None 20. The action of inhibition of acetylcholinesterase enzyme by organophosphorus compounds is through which of the following mechanisms? Reversible competitive inhibition Irreversible competitive inhibition Suicidal inhibition Non-competitive inhibition None 21. Which of the following diagnostic markers is/are incorrectly paired? Ceruloplasmin  - Hepatolenticular degeneration Beta glucocerebrosidase  -  Von Gierke disease High LDH1:LDH2 ratio - Myocardial infarction Lipase  -  Acute pancreatitis 2 2 and 3 1,2,3,4 2,3,4 None 22. Which of the following consumes the highest energy during assimilation? Proteins Mono-unsaturated fatty acids Carbohydrates Poly-unsaturated fatty acids None 23. Which of the following tests will be positive in the urine of a patient who has undergone prolonged starvation?       1                    2                     3 1 1, 3 1,2,3 1,2 None 1 out of 23 Time's up
