Solve Biochemistry INICET 2022 1. Mutation of the gene of cystic fibrosis occurs with: Phenylalanine at 508 position, chloride channel Tryptophan at 708 position, chloride channel Phenylalanine at 708 position, calcium channel Tryptophan at 508 position, calcium channel None 2. A baby is brought to the OPD with complaints of lethargy and poor feeding. On examination, it is noted that the urine had a smell of burnt sugar. A defect in which of the following enzymes causes this disease? Branched-chain amino acid dehydrogenase Branched-chain ketoacid oxidase Branched-chain ketoacid dehydrogenase Branched-chain amino acid oxidase None 3. Which is the correct sequence of steps in the isolation of desirable protein using recombinant DNA technology? SDS PAGE (Sodium dodecyl sulfate-polyacrylate gel electrophoresis) Hybridization Expression of protein and lysis of the bacterial cell Incorporation of genes into bacteria Protein elution 4,1,2,3,5 4,1,3,2,5 4,3,1,2,5 1,2,3,4,5 None 4. All are true about the hexose monophosphate shunt, except: Source of NADPH Source of Acetyl CoA Source of ribose-5-phosphate Utilises ribose None 5. Which of the following techniques is used to detect DNA-protein interaction? DNA fingerprinting DNA footprinting Northern blotting ELISA None 6. Which of the following enzymes uses selenocysteine? Deiodinase Glutathione peroxidase Glutathione synthase Thioredoxin reductase 1,3,4 1,2,4 1,2,3 2,3,4 None 7. A patient presented with reduced levels of high-density lipoprotein, and ABCA1 mutation. On examination, tonsils appeared as shown in the image. What is the diagnosis? Hyperlipoproteinemia Abetalipoprotenemia Tangier's disease Wolman's disease None 8. A patient was diagnosed with Ewing sarcoma with ESWR1 gene mutation. The technique shown in the image was performed to confirm the diagnosis. Identify the technique shown in the image. Karyotyping FISH ( Fluorescent In Situ Hybridisation ) Immunohistochemistry Sanger's technique None 9. A baby is being evaluated for delayed developmental milestones. On examination, the child has hepatosplenomegaly. A microscopic image of the bone marrow evaluation is shown below. What is the diagnosis? Hurler disease Hunter disease Gaucher disease Niemann-Pick disease None 10. Which of the following statements about the human genome project is false? It required international collaboration Total of 20,500 genes were identified Next-generation sequencing was used The project spanned over 13 years None 11. Refsum disease is due to: Absence of alpha oxidation in peroxisomes Absence of alpha oxidation in lysosomes Absence of beta-oxidation in peroxisomes Absence of beta-oxidation in lysosomes None 12. Choose the right sequence of steps in the conversion of cholesterol to testosterone. 17, 20 lyase 20, 22 desmolase 3 beta hydroxy steroid dehydrogenase 17 alpha-hydroxylase 1,2,3,4 2,4,1,3 2,4,3,1 2,3,4,1 None 13. Banding technique used for dicentric chromosomes is: C banding G banding R banding NOR None 14. Which of the following describes the relationship between the melting temperature (Tm) of DNA and the concentration of G-C and A-T? Tm increases with more A-T No relation of GC with Tm Tm increases with more G-C Tm decreases with more G-C None 15. An elderly patient on a vegetarian diet presenting with easy fatigability and pallor was investigated. Peripheral smear showed findings of macrocytic anemia. What is the probable cause of anemia in this patient? Other lab findings are as follows Serum homocysteine - Elevated Serum methylmalonic acid - Normal Hemoglobin - 8 g/dL Mean corpuscular volume (MCV)-120 L Alcohol-induced macrocytosis Folate deficiency Vitamin B12 deficiency Thiamine Deficiency None 16. Affinity maturation of antibodies is because of? Differential mRNA processing Somatic hypermutation Gene rearrangements CD40 None 17. Zellweger syndrome is due to a defect in the biogenesis of: Mitochondria Lysosomes Peroxisomes Nucleus None 18. Best method for the detection of mutations with low allele frequency is FISH Droplet digital PCR Nested PCR Sanger Sequencing None 19. True statement about the action of enhancers of gene expression? Work only when in close conjugation with promoter region They are very specific to the gene Work only when in upstream of promoter region Recruit chromatin and modify regulatory mechanisms None 20. Misincorporation of uracil into DNA is seen in deficiency of: Folate Vitamin B6 Vitamin B12 Vitamin E None 21. Deamination of methylated cytosine forms which of the following? Cytosine Uracil Guanine Thymine None 22. Increased H+ ions in the intermembrane space of mitochondria are due to? Decreased oxidative phosphorylation Increased ATP production Reduced chemiosmosis Water retention in inner mitochondrial space None 23. Fructosamine is obtained by glycation of: Albumin Fructose Urea Glycosaminoglycans None 24. Which of the following types of inhibition is depicted in the given Lineweaver-Burk plot? Non-competitive inhibition Competitive inhibition Suicidal inhibition Uncompetitive inhibition None 25. Glycogen phosphorylase is activated by: Insulin Protein phosphatase Calcium Glucose-6-phosphate None 1 out of 25 Time's up
